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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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Data corrente: |
14/12/2020 |
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Data da última atualização: |
14/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIRO, M. R.; GARCIA, J. M.; LIMA, M. R. de; COLLADO, M. del; SARAIVA, N. Z. |
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Afiliação: |
MARIVALDO RODRIGUES FIGUEIRO, CPATU; Joaquim Mansano Garcia, UNESP; Marina Ragagnin de Lima, UNESP; Maite del Collado, USP; NAIARA ZOCCAL SARAIVA, CNPGL. |
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Título: |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes? |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Zygote, v. 28, n. 2, p. 109-115, Apr. 2020. |
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DOI: |
https://doi.org/10.1017/S096719941900073X |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. MenosIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 ... Mostrar Tudo |
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Palavras-Chave: |
Desenvolvimento embrionário; Soro fetal bovino. |
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Thesagro: |
Búfalo; Lipídio. |
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Thesaurus Nal: |
Buffaloes; Lipid metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02374naa a2200253 a 4500
001 2128094
005 2020-12-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S096719941900073X$2DOI
100 1 $aFIGUEIRO, M. R.
245 $aIs it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?$h[electronic resource]
260 $c2020
520 $aIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.
650 $aBuffaloes
650 $aLipid metabolism
650 $aBúfalo
650 $aLipídio
653 $aDesenvolvimento embrionário
653 $aSoro fetal bovino
700 1 $aGARCIA, J. M.
700 1 $aLIMA, M. R. de
700 1 $aCOLLADO, M. del
700 1 $aSARAIVA, N. Z.
773 $tZygote$gv. 28, n. 2, p. 109-115, Apr. 2020.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 45 | |
| 3. |  | FALLGATTER, J.; SOUZA, A. L. de; CAMARGO, D.; BARRETO, G. U.; BEHLING, M. O desbaste das árvores de eucalipto reduz a perda de produtividade da soja na iLPF? In: ENCONTRO DE CIÊNCIA E TECNOLOGIAS AGROSSUSTENTÁVEIS, 3.; JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 8., 2019, Sinop. Resumos... Brasília, DF: Embrapa, 2019. p. 29.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 10. | | SOUZA, S. F.; PEREIRA, A. F. C.; LIMA, J. R. F. de; SOUZA, A. L. de. Transmissão de preços da manga brasileira entre os mercados interno, americano e europeu. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 56., 2018, Campinas. Transformação recentes na agropecuária brasileira: desafios em gestão, inovação, sustentabilidade e inclusão social. Campinas: UNICAMP, 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 12. | | BEHLING, M.; CARVALHO, G. de; SOUZA, A. L. de; CAMARGO, D.; FALLGATTER, J. Horizontal distribution of the soybean yield in integrated crop-livestock-forest system in the north of Mato Grosso, Brazil. Pesquisa Florestal Brasileira, v. 39, e201902043, p. 495, 2019. Special issue. Abstracts of the XXV IUFRO World Congress.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 15. | | FREITAS, D. de G. C.; CANDÉA, T. V.; MATTA, V. M. da; SOUZA, A. L. de; PONTES, S. M. Aceitabilidade de abacaxi (Ananas comosus L. Merril) em calda de melado de cana. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Potencialidades, desafios e inovações. [S.l.]: SBCTA, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 16. | | FREITAS, D. de G. C.; NEVES, A. C. V.; SOARES, A. G.; SOUZA, A. L. de; BARBOZA, H. T. G. Aceitação sensorial de geleia de caqui (Diospyros kaki L.). In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Potencialidades, desafios e inovações. [S.l.]: SBCTA, 2010. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 17. | | DRUMOND, M. A.; BARROS, N. F. de; SOUZA, A. L. de; SILVA, A. F. da; MEIRA NETO, J. A. A. Alteracoes fitossociologicas e edaficas na Mata Atlantica em funcao das modificacoes da cobertura vegetal. Revista Arvore, Vicosa, MG, v. 20, n. 4, p. 451-466, 1996.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | BASSO, V. M.; JACOVINE, L. A. G.; GRIFFITH, J. J.; NARDELLI, A.; ALVES, R. R.; SOUZA, A. L. de. Programas de fomento rural no Brasil. Pesquisa Florestal Brasileira, Colombo, v. 32, n. 71, p. 321-334, jul./set. 2012. Artigo de revisão.| Biblioteca(s): Embrapa Florestas. |
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| 19. | | DRUMOND, M. A.; BARROS, N. F. de; SOUZA, A. L. de; SILVA, A. F. da; TEIXEIRA, J. L. Composição mineral e demanda nutricional de espécies florestais da Mata Atlântica. Revista Arvore, Viçosa, v. 21, n. 1, p. 1-10, 1997.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| 20. | | OLIVEIRA, M. L. R. de; LEITE, H. G.; NOGUEIRA, G. S.; GARCIA, S. L. R.; SOUZA, A. L. de. Classificação da capacidade produtiva de povoamentos não desbastados de clones de eucalipto. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 11, p. 1559-1567, nov. 2008. Título em inglês: Classifi cation of the productive capacity of unthinned stands of eucalypt clones.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 45 | |
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